VEGF-165, human recombinant

VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, it plays a prominent role in normal and pathological angiogenesis.   StemRD’s VEGF-165 is made in human 293 cells as a 45kDa glycoprotein consisting of two 165 amino acid polypeptide chains. It is purified by aseries of chromatography.

Description:

VEGF is a potent growth and angiogenic cytokine. It stimulatesproliferation and survival of endothelial cells, and promotes angiogenesisand vascular permeability. Expressed in vascularized tissues, it plays aprominent role in normal and pathological angiogenesis.StemRD’s VEGF-165 is made in human 293 cells as a 45kDa glycoproteinconsisting of two 165 amino acid polypeptide chains. It is purified by aseries of chromatography.

Formulation   Lyophilized in sterile filtered solution of PBS.

Reconstitution

Before reconstitution, a brief spin is recommend to drive down any materialdislodged from the bottom of the tube. The lyophilized protein should bereconstituted in sterile H2O to a desired concentration.

Stability

The lyophilized protein is stable for at least one year if stored at -80 degreeC. Reconstituted protein is stable for at least four weeks at 4 degree C, butshould be stored in aliquots at -80 degree C for longer term. Avoidrepeated freeze and thaw.

Purity   Greater than 95% as determined by SDS-PAGE analysis and visualized bysilver staining.

Biological Activity The activity was determined by using a HUVEC cells. The ED50 rangesfrom 2- 10 ng/ml. Activity in other assays should be determined by eachindividual setting.

Country of Origin   USA  

For Research Use Only. Not for Use in Human.