LIF(Leukemia Inhibitory Factor), mouse recombinant

Catalog #： LIF-010; LIF-050;LIF-250; LIF-1000

Description ：

LIF derives its name from its ability toinduce the terminal differentiation of myeloid leukaemic cells. Otherproperties attributed to the cytokine include: the growth promotion and celldifferentiation of different types of target cells, influence on bonemetabolism, cachexia, neural development, embryogenesis and inflammation.

LIF has been used in mouse embryonic stemcell culture to maintain the stem cells in an undifferentiated state, however,LIF is not required for culture of human embryonic stem cells.

StemRD produces LIF in soluble form from E.coli as a single-chain polypeptide containing 181 amino acids with a molecularmass of 20 kDa. This non-tagged protein is purified by a series ofchromatography.

Source ：Escherichia coli.Endotoxin level of this product is lower than 0.1 EU/ug.

Formulation ：Lyophilized fromsterile filtered PBS solution, carrier-free.

Reconstitution： Beforereconstitution, we recommend a brief spin to drive down any material dislodgedfrom the bottom of the tube. It is recommended to reconstitute the lyophilizedproduct in sterile water to no less than 100ug/ml. Further dilutions should bemade in sterile buffer or medium containing carrier proteins, such as albuminor serum.

Stability ：The lyophilizedprotein is stable for at least 1 year if stored at -80 degree C. Reconstitutedprotein is stable for at least 2 weeks at 4 degree C, but should be stored inaliquots at -80 degree C for longer term. Avoid repeated freeze/thaw.

Purity： Greater than 98% bySDS-PAGE analysis

Biological Activity ：The activitywas determined by a STAT3 reporter gene assay in rodent

cells. The EC50 ranges from 0.3 – 3.0ng/mL.

For Research Use Only. Not for Use in Humans.