UltraCULTURETM Serum-free Medium

Catalog #:   12-725F

UltraCULTURETM Serum-free medium, without L-Glutamine, 500 mL

Product Overview

UltraCULTURE^TM Serum-free Medium is a complete, allpurpose medium designed for the cultivation of a wide variety of adherent andnon-adherent mammalian cell types. UltraCULTURE^TM Medium can beused to support fusion of cells during hybridoma formation, growth of monocyte,macrophage, epithelial, and fibroblastic cell lines.

The medium is supplemented with recombinant human insulin, bovinetransferrin, and a purified mixture of bovine serum proteins. The total proteinconcentration of UltraCULTURE^TM Medium is approximately 3mg/ml.

UltraCULTURE^TM Medium can be supplemented withCryoprotective Medium (Cat. No. 12-769E) to cryopreserve cells in a serum-freeenvironment.

UltraCULTURE^TM Medium does not contain L-glutamine; pleaseadd 5 ml of 200 mM L-glutamine solution (Cat. Nos. 17-605 or 17-905) prior touse.

The formulation for UltraCULTURE^TM Medium has beensubmitted to the FDA as a Master File. Permission to cross reference the MasterFile may be obtained by contacting the Regulatory Affairs Department.

Turbidity may develop in UltraCULTURE^TM Medium, qualitytests have determind that turbidity will not alter the performance of theproduct.

All BioWhittaker^TM Cell Culture Media products are forResearch Use Only (RUO) and are not approved for human or veterinary use or foruse in clinical or in vitro diagnostic procedures. If you require GMP grademedia, contact Lonza for more details.

Benefits

General purpose medium to replace Serum

Applications

Cultivation ofadherent and non-adherent mammalian cells

Generation ofviral particles for vaccine production

Clinicalimmunotherapy applications