产品中文名称：重组人 LIF
产品英文名称：Peprotech 300-05-5 Recombinant Human LIF
Peprotech重组人 LIF产品编号:

300-05
Peprotech重组人 LIF别名:

Leukemia Inhibitory Factor, Differentiation-stimulating factor, D factor,Melanoma-derived LPL inhibitor (MLPLI)
Peprotech300-03-100 Recombinant Human LIF描述:

LIF is a pleiotrophic factor produced by multiple cell types, including Tcells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIFpromotes long-term maintenance of embryonic stem cells by suppressingspontaneous differentiation. Other activities include the stimulation of acutephase protein synthesis by hepatocytes, stimulation of differentiation ofcholinergic nerves, and suppression of adipogenesis by inhibiting thelipoprotein lipase in adipocytes. While human LIF is active on mouse cells andis widely used in the maintenance of murine ESC to prevent spontaneousdifferentiation, mouse LIF is not active on human cells due to its inability tobind to the human LIF receptor. Recombinant Human LIF is a 19.6 kDa proteincontaining 180 amino acids residues, including three disulfide bonds.
Peprotech 重组人 LIF氨基酸序列:

SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPFHANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVGHVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

Peprotech重组人 LIF来源:

E.coli

Peprotech重组人 LIF纯度:
Greater than 98% by SDS-PAGE gel and HPLC analyses.

Peprotech重组人 LIF生物活性:
Determined by its ability to stimulate the proliferation of human TF-1 cells.The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x107 units/mg.

Peprotech重组人 LIF交叉反应:
Mouse, Human

Peprotech重组人 LIF描述：
LIF是由多种细胞类型，包括T细胞，骨髓单核细胞谱系，成纤维细胞，肝，心脏和黑素瘤产生的多效因子。 LIF通过抑制自发分化促进长期维持胚胎干细胞。其他活动包括急性期蛋白合成的刺激肝细胞，胆碱能神经分化的刺激和抑制脂肪形成的抑制脂肪细胞的脂蛋白脂酶。虽然人类LIF是活性对小鼠细胞和广泛应用于鼠ESC的维护，以防止自发分化，鼠标LIF没有激活人体细胞，由于其不能结合人LIF受体。重组人LIF是一种含有180个氨基酸残基，其中包括三个二硫键19.6 kDa蛋白。