重组SARS-CoV-2 S蛋白RBD（Fc Tag），GMP级

英文名称：Recombinant SARS-CoV-2 Spike Protein-RBD(Fc Tag)

产品货号：GMP-TL762

♦   产品描述（Description）

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

♦   分子别名（Synonym）

2019-nCoV RBD Protein; 2019-nCoV S1 Protein; 2019-nCoV Spike RBD Protein

♦   表达宿主（Expression host）

CHO细胞

♦   蛋白序列（Protein Sequence）

DNA序列编码重组SARS-CoV-2 (2019-nCoV)RBD蛋白表达带有Fc标签在C末端

♦   分子量（Molecular Weight）

重组SARS-CoV-2 (2019-nCoV) RBD蛋白包含470个氨基酸，预测的理论分子量为52.9kd

♦   纯度（Purity）

≥95%采用SDS-PAGE凝胶分析和SEC-HPLC分析

♦   内毒素（Endotoxin）

≤0.01EU/μg（凝胶法）

♦   提示（Tips）

本蛋白带有人Fc标签，应用中会与人源抗体的二抗发生反应，因此请特别注意

♦   提纯方法（Purification Method）

层析纯化

♦   稳定性（Stability）

1、冻干的样本可在4℃保存24个月，溶解后的液体可于-20℃保存6-12个月，并且避免反复冻融。

2、液体样本在-20～-80℃保存12个月，融化后需要进行分装保存于-20℃保存6-12个月，避免反复冻融。