重组SARS-CoV-2棘突蛋白（S1亚基），GMP级

英文名称：Recombinant SARS-CoV-2 Spike Protein(S1 Subunit)

产品货号：GMP-TL761

♦   产品描述（Description）

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-Cov-2   can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

♦   分子别名（Synonym）

2019-nCoV coronavirus spike Protein; 2019-nCoV cov spike Protein; ncov RBD Protein, 2019-nCoV; 2019-nCoV ncov s1 Protein; 2019-nCoV NCP-CoV s1 Protein;

♦   表达宿主（Expression host）

人HEK293细胞

♦   蛋白序列（Protein Sequence）

DNA序列编码重组SARS-CoV-2 (2019-nCoV) S1蛋白表达带有His标签在C末端

♦   分子量（Molecular Weight）

重组SARS-CoV-2 (2019-nCoV) S1蛋白包含698个氨基酸，预测的理论分子量为78.3kd

♦   纯度（Purity）

＞90% 采用SDS-PAGE凝胶分析

♦   内毒素（Endotoxin）

≤0.01EU/μg（凝胶法）

♦   图片（Images）

♦   稳定性（Stability）

1、冻干的样本可在4℃保存24个月，溶解后的液体可于-20℃保存6-12个月，并且避免反复冻融。

2、液体样本在-20~-80℃保存12个月，融化后需要进行分装保存于-20℃保存6-12个月，避免反复冻融。

♦   组成（Component）

1.   含有6%甘露醇和6%海藻糖的无菌PBS（pH7.4）的冻干粉。  

2.   0.2 μM 无菌过滤的PBS,pH 7.4的液体。  

   注意：所有产品“仅用于非临床科研用途，不用于诊断、治疗、临床或其他用途”！